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Only homozygous animals were being Utilized in the analyses, regardless of whether heterozygous mice were operate in behavioral experiments. Three cohorts of 11–16 mice underwent the 1st battery of behavioral assays for a complete of 42 animals. All mice ended up in the beginning housed with their littermates of the identical sexual intercourse inside the Vanderbilt Murine Neurobehavioral Core, with managed 12 h light-weight/dim cycles, freely available foods and water, and controlled temperature and humidity. The experimenter was at first blinded into the genotype of every animal, and each animal underwent the same battery of behavioral exams to establish phenotype.
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